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All iPSC cultures were maintained on Matrigel TM (Corning) coated 60 mm tissue culture dishes (Greiner Bio-One, Austria) in mTeSR-1 TM medium (Stemcell Technologies, Canada) in a humidified incubator (Thermo, Australia) at 37 C and 5% O 2

Samenius proposed that MC is more frequently observed in patients with an obstruction anywhere in the colon and rectum than in cases of chronic constipation where there is no mechanical cause for the constipation.8 A list of the etiologies of MC is shown in Fig

Try to exercise for at least 30 minutes a day, five times a week

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Site Rotation: Rotate injection sites systematically (abdomen, thighs, upper arms) to prevent tissue irritation

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