Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
Yes, provided it is purchased strictly as a reference compound for in-vitro laboratory research and not intended for human consumption, operating in strict compliance with SFDA research material importation guidelines
After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
GLP-1 receptor agonists are medications that mimic the natural hormone, allowing it to remain active in the body longer and deliver powerful health benefits
Mills KT, et al
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