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Once the brown preadipocytes reached 7080% confluence, they were infected with retroviral particles encoding SV40 Large T antigen in culture medium containing 20% heat-inactivated fetal bovine serum (FBS) and polybrene (8 g/mL) for 48 h
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In summary , to treatment with GLP1-RAs compared with placebo or active controls was associated with increased risk of the composite outcome of gallbladder or biliary diseases and for cholelithiasis, cholecystitis, and biliary diseases
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