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Search Strategy A comprehensive literature search was conducted using the National Library of Medicine database (www.pubmed.ncbi.nlm.nih.gov ) without applying any language or publication date restrictions

Bioinformatics analysis of genes related to iron death in diabetic nephropathy through network and pathway levels based approaches

Alcian blue staining solution was applied for a duration of 10 to 20 min, followed by three washes with distilled water, each lasting 1 to 2 min

This reduces amino acid recycling, raises blood acidity, and depletes folate, worsening the deficiency

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It is made of three essential amino acids (cysteine, glutamate, and glycine)